BCIP / NBT Color Development Substrate, 100 ml

BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Substrate Solution is a chromogenic substrate for alkaline phosphatase (AP) enzymes, widely used in molecular biology and immunology. This substrate produces an intense purple precipitate upon reaction with AP, making it ideal for applications that require clear, visual detection. BCIP/NBT is commonly used in Western blotting, ELISA, and in situ hybridization to detect target proteins or nucleic acids. The BCIP/NBT Substrate Solution is a stable, ready-to-use mixture optimized for high sensitivity in AP-based detection systems. BCIP serves as the substrate, while NBT acts as the colour development reagent. This combination allows for the formation of a durable purple precipitate upon enzyme activation, offering excellent contrast and ease of visualization for accurate analysis.