Tris-Borate-EDTA (TBE) buffer is widely utilised in DNA agarose gel electrophoresis as the gel matrix and running buffer. It is particularly advantageous for high-resolution separation of small DNA fragments and is often preferred when consistent buffer performance is required over prolonged electrophoresis runs. TBE is known for maintaining buffer integrity over extended periods, which is essential in applications where reproducibility and accuracy are crucial.