Tris-Glysine-SDS Running Buffer, 10X

Tris-Glycine SDS Running Buffer is a staple in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), used for the separation of proteins based on their molecular weight. This buffer, provided in a convenient pre-mix powder form, ensures precise and consistent results. The buffer is essential for denaturing electrophoresis, where proteins are uniformly separated in the gel matrix. The Tris-Glycine SDS Running Buffer is composed of Tris base, glycine, and SDS (Sodium Dodecyl Sulfate). Once dissolved in distilled water, it provides a stable pH environment (approximately 8.3) crucial for the denaturation and uniform migration of proteins during SDS-PAGE. The pre-mix powder is designed for convenience, ensuring that the buffer can be prepared quickly and accurately, reducing preparation time and minimizing errors.