Tris-Acetate-EDTA (TAE) buffer is frequently utilized in DNA agarose gel electrophoresis, both within the gel and as the running buffer. Compared to Tris-Borate-EDTA (TBE) buffer, TAE allows for faster separation of linear and double-stranded DNA. It is particularly recommended for preparative gel electrophoresis where separated DNA fragments will be used in cloning or other processes involving enzymatic treatments.