Product

NexGen HM™ Protease Inhibitor delivers powerful, broad-spectrum protection against unwanted proteolytic activity during cell lysis and protein isolation. Its non-toxic formulation effectively inhibits 16+ proteases across multiple classes, helping prevent protein degradation, preserve sample integrity, and improve protein recovery.

Tris-Glycine SDS Running Buffer is a staple in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide
Gel Electrophoresis), used for the separation of proteins based on their molecular weight.

Tris-EDTA (TE) buffer is commonly used in molecular biology laboratories for the preparation, storage,
and protection of nucleic acid samples. It provides a stable alkaline environment that helps maintain the
integrity of DNA and RNA during experimental procedures.

Tris-Borate-EDTA (TBE) buffer is widely utilised in DNA agarose gel electrophoresis as the gel matrix
and running buffer. It is particularly advantageous for high-resolution separation of small DNA
fragments and is often preferred when consistent buffer performance is required over prolonged
electrophoresis runs.

Tris-Acetate-EDTA (TAE) buffer is frequently utilized in DNA agarose gel electrophoresis for running
and preparation of the gel. TAE allows for faster separation of linear and double-stranded DNA.

Tris-Acetate-EDTA (TAE) buffer is frequently utilized in DNA agarose gel electrophoresis for running
and preparation of the gel. TAE allows for faster separation of linear and double-stranded DNA.

SDS-PAGE Loading Dye is used for preparing protein samples for sodium dodecyl sulfate-polyacrylamide
gel electrophoresis (SDS-PAGE). This dye contains SDS, which imparts a negative charge to proteins, and
other components that facilitate sample loading and visualization.

RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer is a versatile and powerful detergent-based buffer
used for the extraction of total protein, including cytoplasmic, membrane-bound, and nuclear proteins,
from various types of tissues and cultured cells.

PMSF (Phenylmethylsulfonyl Fluoride) is a potent serine protease inhibitor used to prevent proteolytic degradation of proteins during extraction and purification

PMSF (Phenylmethylsulfonyl Fluoride) is a potent serine protease inhibitor used to prevent proteolytic
degradation of proteins during extraction and purification.

Phosphate-buffered saline (PBS) is a widely used buffer in cell culture due to its isotonic and non-toxic
nature. It maintains a stable pH environment compatible with cellular functions and closely replicates the
osmolarity of human blood, ensuring cell integrity during various laboratory procedures.

Phosphate-buffered saline (PBS) is a widely used buffer in cell culture due to its isotonic and non-toxic
nature. It maintains a stable pH environment compatible with cellular functions and closely replicates the
osmolarity of human blood, ensuring cell integrity during various laboratory procedures.

NP-40 Tissue Lysis Buffer is a non-denaturing detergent buffer commonly used for the extraction of
proteins, nucleic acids, and other macromolecules from tissues and cells. This buffer is specifically
formulated to gently disrupt cell membranes while preserving the native structure and biological activity
of proteins. NP-40, a nonionic surfactant, is the key component that facilitates efficient lysis of cellular
membranes without denaturing the proteins, making this buffer ideal for applications requiring intact
protein complexes or enzymatic activity.

NP-40 Tissue Lysis Buffer is a non-denaturing detergent buffer commonly used for the extraction of
proteins, nucleic acids, and other macromolecules from tissues and cells. This buffer is specifically
formulated to gently disrupt cell membranes while preserving the native structure and biological activity
of proteins. NP-40, a nonionic surfactant, is the key component that facilitates efficient lysis of cellular
membranes without denaturing the proteins, making this buffer ideal for applications requiring intact
protein complexes or enzymatic activity.

NexGen Proteinase K is a highly efficient serine protease that exhibits broad substrate specificity. It is
commonly used for the digestion of proteins, including those resistant to other proteases, and is especially
effective in removing nucleases from DNA and RNA preparations.

Introduction:
NexGen HM RIPA (Radio-Immunoprecipitation Assay) Buffer with Protease Inhibitor is a powerful and
versatile lysis buffer designed for the efficient extraction of total proteins from various cell and tissue types.
This buffer is pre-formulated with a robust protease inhibitor, providing broad-spectrum protection against
proteolytic degradation.

NexGen HM Protease Inhibitor for Tissue Lysate is a highly potent inhibitor designed to protect proteins
from proteolytic degradation during tissue lysis. This inhibitor mix is optimized for use in tissue lysates,
providing broad-spectrum inhibition of serine, cysteine, and metalloproteases.

NexGen HM Protease Inhibitor for Mammalian Cell Lysate is a premium inhibitor formulated to prevent
proteolytic degradation during the lysis of mammalian cells. It’s providing broad-spectrum inhibition of
serine, cysteine, metalloproteases, aspartic, glutamic & threonine classes of protease enzyme ensuring the
preservation of protein integrity during cell lysis.

The Biopioneer Bradford reagent is a quick and well-known Bradford Coomassie-binding,
colorimetric method for total protein quantitation. When Coomassie dye binds protein in an
acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with
a concomitant color change from brown to blue.

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally
stable under stable environmental conditions. However, protein degradation is enhanced when cells are
studied invitro.

Source : Escherichia Coli. Fibroblast Growth Factor 23 Human Recombinant produced in
E.Coli is a single, non glycosylated polypeptide chain containing a total of 228 amino acids
and having a molecular mass of 22.5kDa. The FGF 23 is and purified by chromato graphic
techniques. FGF