Research and Laboratory

BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Substrate Solution is a chromogenic substrate for alkaline phosphatase (AP) enzymes, widely used in molecular biology and immunology. This substrate produces an intense purple precipitate upon reaction with AP, making it ideal for applications that require clear, visual detection. BCIP/NBT is commonly used in Western blotting, ELISA, and in situ hybridization to detect target proteins or nucleic acids. The BCIP/NBT Substrate Solution is a stable, ready-to-use mixture optimized for high sensitivity in AP-based detection systems. BCIP serves as the substrate, while NBT acts as the colour development reagent. This combination allows for the formation of a durable purple precipitate upon enzyme activation, offering excellent contrast and ease of visualization for accurate analysis.

NexGen Proteinase K is a highly efficient serine protease that exhibits broad substrate specificity. It is commonly used for the digestion of proteins, including those resistant to other proteases, and is especially effective in removing nucleases from DNA and RNA preparations. This enzyme works over a wide range of temperatures and pH levels, making it versatile for various molecular biology applications. NexGen Proteinase K is a robust enzyme that can degrade a wide variety of proteins, including native proteins, during nucleic acid purification processes. It is active in the presence of SDS, urea, and other detergents, allowing it to function in complex lysis buffers. NexGen Proteinase K is available in both liquid and lyophilized forms, ensuring compatibility with different experimental needs.

NP-40 Tissue Lysis Buffer is a non-denaturing detergent buffer commonly used for the extraction of proteins, nucleic acids, and other macromolecules from tissues and cells. This buffer is specifically formulated to gently disrupt cell membranes while preserving the native structure and biological activity of proteins. NP-40, a nonionic surfactant, is the key component that facilitates efficient lysis of cellular membranes without denaturing the proteins, making this buffer ideal for applications requiring intact protein complexes or enzymatic activity. NP-40 Tissue Lysis Buffer is widely used in various biochemical and molecular biology applications, including protein purification, immunoprecipitation, and Western blotting. NP-40 Tissue Lysis Buffer is composed of NP-40 (Nonidet P-40), a non-ionic detergent, along with other buffering agents that maintain a stable pH environment during lysis. The buffer is designed to lyse cells effectively, solubilize membrane proteins, and release cytoplasmic and nuclear contents while minimizing the risk of protein degradation or denaturation. This makes it suitable for the extraction of functional proteins, protein complexes, and nucleic acids from tissue samples, cultured cells, and other biological materials.

NP-40 Tissue Lysis Buffer with Protease Inhibitor is a pre-formulated, ready-to-use buffer designed for effective cell and tissue lysis, ensuring high protein yield and activity preservation. The inclusion of protease inhibitors safeguards proteins from degradation during extraction, making it ideal for applications in Western blotting, enzyme assays, and protein quantification. This NP-40 Tissue Lysis Buffer is a 1X solution containing NP-40, a nonionic detergent, for gentle yet effective lysis of cellular membranes, allowing for the release of cytoplasmic, nuclear, and membrane-bound proteins. The buffer also includes a blend of protease inhibitors to prevent proteolytic degradation, preserving protein function and integrity. The solution is sterile filtered to support compatibility with cell culture and sensitive proteomic workflows.