NP-40 Tissue Lysis Buffer, 10X

NP-40 Tissue Lysis Buffer is a non-denaturing detergent buffer commonly used for the extraction of proteins, nucleic acids, and other macromolecules from tissues and cells. This buffer is specifically formulated to gently disrupt cell membranes while preserving the native structure and biological activity of proteins. NP-40, a nonionic surfactant, is the key component that facilitates efficient lysis of cellular membranes without denaturing the proteins, making this buffer ideal for applications requiring intact protein complexes or enzymatic activity. NP-40 Tissue Lysis Buffer is widely used in various biochemical and molecular biology applications, including protein purification, immunoprecipitation, and Western blotting. NP-40 Tissue Lysis Buffer is composed of NP-40 (Nonidet P-40), a non-ionic detergent, along with other buffering agents that maintain a stable pH environment during lysis. The buffer is designed to lyse cells effectively, solubilize membrane proteins, and release cytoplasmic and nuclear contents while minimizing the risk of protein degradation or denaturation. This makes it suitable for the extraction of functional proteins, protein complexes, and nucleic acids from tissue samples, cultured cells, and other biological materials.